Sperm and scrambled eggs

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Sperm and scrambled eggs

A newspaper obituary not long ago revealed that its subject was believed to have been both the son and grandson of the lecherous monarch, Edward VII. (Work it out for yourself.) But this is as nothing to the conundrums that Lee Silver sets out in his mesmerizing vision of our genetic future. Within weeks of the eruption of Dolly the sheep into the pages of Nature — and regardless of the doubts ...

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Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio)

Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated samp...

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Sperm Receptivity in Sea Urchin Oocytes and Eggs

An electrophysiological method for studying the receptivity to spermatozoa of sea urchin eggs and oocytes is described. Pairs of adjacent oocytes and eggs, with intact jelly layers, were impaled and simultaneously exposed to a known concentration of spermatozoa. Two parameters were studied. The time from insemination to the first successful collision (as indicated by a step depolarization acros...

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Generation of functional eggs and sperm from cryopreserved whole testes.

The conservation of endangered fish is of critical importance. Cryobanking could provide an effective backup measure for use in conjunction with the conservation of natural populations; however, methodology for cryopreservation of fish eggs and embryos has not yet been developed. The present study established a methodology capable of deriving functional eggs and sperm from frozen type A spermat...

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Soluble sperm factors and Ca2+ release in eggs at fertilization.

All eggs are activated at fertilization by an increase in intracellular free Ca2+. How the spermatozoa triggers the release of intracellular Ca2+ has not been established in any species. One hypothesis is that spermatozoa introduce a Ca(2+)-releasing factor into the cytoplasm after gamete membrane fusion. It has been suggested that spermatozoa may introduce Ca2+ itself, or a small molecular wei...

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ژورنال

عنوان ژورنال: Current Biology

سال: 1998

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(98)70188-5